Summary
Type: SPE
Application #: 305200
Catalog#: 730748 - SPE Cartridges, Chromabond, HR-XAW, 3mL, 200mg, 30/pk
Detection:
Conditions: Column conditioning: 5 ml methanol, then 5 ml water
(Do not let the column run dry!)
Sample aspiration: The sample is passed through the column by vacuum or pressure (max. 1000 ml sample volume).
Washing 1: 25 mM ammonium acetate in water
Washing 2: / Elution 1: 2 ml methanol (elution of neutral and basic compounds)
Drying: With nitrogen or air
Elution 2: 2 x 2 ml 1 - 5 % ammonia in methanol (elution of strongly acidic compounds)
(Acidic methanol (formic acid) can be used alternatively for elution 2. Here an interruption of the interactions with the anion exchanger results by a protonation of the analyte.)
Evaporation and reconstitution (if necessary)
Authors: SPE department
Source: Macherey-Nagel, Germany 2009